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Development of Biofungicides and Biofertilizers

To support sustainable agriculture, we identify, enhance and develop novel products for the promotion of plant growth, tolerance of abiotic stress and control of plant diseases.

Image: FEM 71

Instruments

Image: Stereomicroscopi
Microscopes

Instruments used for microbiological analysis associated to acquisition devices and software for image editing. 

 
Image: Real time PCR
Thermocyclers and real time PCR

Analysis of acid nucleic for amplification and quantification of microorganisms and gene expression 

Image: IMG_4814
Greenhouse

Eight independent chambers allow for experimentation on plant growth under controlled conditions (temperature and humidity) 

Image: CAMERE DI CRESCITA DA 700 L
Climatic chambers

700 l chambers in which leaf disks and in vitro tests are performed under controlled conditions 

Image: Simulatore di pioggia
Rain simulator

It simulates rainfall conditions in order to test the rainfastness of a formulation under controlled conditions.

Image: Fermentatore
Bioreactor

Device (5 l) for the simulation of industrial production of biological control agents.

Services

Evaluation of characteristics for the development of biocontrol agents

Tests to evaluate mass production, phytotoxicity, substrate growth suitability, resistance to desiccation, UV radiation and washout.  

Evaluation of biofungicide formulations in controlled conditions (no GEP)

Tests on efficacy, persistence and rainfastness of biofungicide formulations (No GEP) in vitro or in planta (leaf disk or greenhouse conditions) against the main grapevine or apple tree pathogens.

Efficacy evaluation of biofungicide formulations under semi-field and in field (no GEP) 

Tests of the efficacy of biofungicide formulations subjected to the environmental factors such as rainfall, sunlight, air humidity, temperatures, under semi-field and field conditions.

Evaluation of control activity against plant pathogens

Test on 1) plant protection and induced systemic resistance, 2) defence gene induction tests, 3) biofilm formation, and production of antibiotics, volatile and non-volatile composts.

Evaluation of plant-growth-promoting activities

Test on 1) potassium and phosphate solubilization, siderophore production, indole-3-acetic acid and ACC-deaminase production; 2) nitrogen fixing and plant-growth-promoting under climatic chamber or greenhouse conditions.

Microbiological analysis of soil and compost

Quantification of culturable microorganisms living in soil and compost samples, including fungal and bacteria diversity, thermophilic and psychrophilic, pathogen and antagonist microorganisms. Fungal and bacterial diversity using amplicon sequencing.

Microorganisms collection

1) Isolation, identification and conservation of microorganisms isolated from diverse substrates, 2) screening of strains of interest in phytopathology, biotechnology and agro-industry.

Applications

Biological control

Development and strengthening of new biofungicides and biofertilisers that meet the needs of organic and integrated farms on the reduction of chemicals and for a more sustainable agriculture.

Soil biological management

Soil microbiome analysis as a support for the individuation of agricultural practices that promote crop productivity and maintain the balance of microbial communities and soil health.