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Fondazione Edmund Mach
Fondazione Edmund Mach
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Metabolomics

We use innovative methodologies for the quantification and characterization of metabolites present in complex matrices. Our analytical techniques are applicable to plant, human, animal, and cell models.

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Instruments

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UPLC-Q-TOF SYNAPT (High Resolution Mass Spectrometer)

Two high performance liquid chromatographs coupled to high resolution mass spectrometers for untargeted analysis of primary and secondary metabolites in plant matrices 

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HPLC-ORBITRAP XL (High Resolution Mass Spectrometer)

High performance liquid chromatograph coupled to orbitrap mass spectrometer for untargeted analysis of primary and secondary metabolites in human/animal samples

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UHPLC-MS/MS

Two high performance liquid chromatographs coupled to triple quadrupole mass spectrometers for the quantification of primary and secondary metabolites 

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UHPLC-Qtrap

Two high performance liquid chromatographs coupled to triple quadrupole mass spectrometers with linear trap for the quantification of primary and secondary metabolites.

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HPLC-DAD and HPLC-DAD-MS

High performance liquid chromatograph coupled to UV-VIS detector and single quadrupole mass spectrometer for the quantification of secondary metabolites.

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GCxGC TOF

Two-dimensional gas chromatograph coupled to a time-of-flight mass spectrometer for untargeted analysis of VOCs

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GC-MS/MS

Two gas chromatographs coupled to triple quadrupole mass spectrometers for the quantification of primary metabolites and VOCs

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GC-O-MS

Gas chromatograph coupled to single quadrupole mass spectrometer and olfactometer for the analysis and identification of sensory important VOCs

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Fast-GC-MS/MS

Fast-gas chromatograph coupled to triple quadrupole mass spectrometer for the quantification of primary metabolites and VOCs

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Other equipment

Other instrumentation for analysis (e.g. spectrophotometer / colorimeter), for sample preparation (e.g. anoxic chamber) and for compound isolation (preparative HPLC).

Services

Quantitative analysis of secondary metabolitesQuantification of different classes of secondary metabolites through LC and GC techniques coupled with mass spectrometers (polyphenols, anthocyanins, VOCs ...)
Quantitative analysis of primary metabolitesQuantification of different classes of primary metabolites through LC and GC techniques coupled to mass spectrometers (Organic acids, carbohydrates, amino acids, SCFA, lipids ...)
Untarget analysis of metabolites on plant samplesProfiling of different classes of metabolites through LC and GC techniques coupled to high resolution mass spectrometers, on different plant material (food, plants, wine, other)
Untarget analysis of metabolites on human and animal samplesThe platform offers the possibility to carry out the profiling of different classes of metabolites through LC and GC techniques coupled to high resolution mass spectrometers (nutritional studies and nutrikinetics of bioactive compounds).
Olfactometric gas chromatographic analysisThe platform offers the possibility to carry out olfactometric studies after gas chromatographic separation to correlate the aroma to a specific compound present in the matrix.
Isolation of compounds of interest from complex matricesThe platform offers the possibility to isolate specific compounds of interest from complex matrices, through optimized extractions and preparative chromatography.

Applications

MetabolomicsThe platform uses methods based on mass spectrometry to identify and quantify molecules in different biological matrices and plant extracts, for metabolomics and lipidomics studies.
Viticulture and enologyThe platform offers analysis of metabolites present in oenological products; in viticulture in the study metabolomic analysis of plant-pathogen interactions, using quantitative and screening techniques
AgrifoodThe platform offers both fingerprinting analyzes (analysis of all measurable analytes to identify differences) and targeted analyzes (quantification of a set of analytes) on different agri-food products.
Food and nutritionThe platform offers metabolomic analyzes of active compounds in humans (within acute and chronic intervention, nutritional studies conducted externally), and studies of metabolism and transport of metabolites in model animals.