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Fondazione Edmund Mach
Fondazione Edmund Mach
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Sequencing and Genotyping

We provide seamless access to Next Generation Sequencing technologies and high-density SNP array analysis, developing innovative protocols in the agricultural, environmental and food science fields.

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Instruments

Image: MISEQ ILLUMINA
MISEQ ILLUMINA

NGS sequencing platform that allows to generate up to 25 million reads from 25 to 300 nucleotides in paired end mode (PE 2X300) 

Image: MinION ONT
MinION ONT

Portable real time device for DNA and RNA sequencing by nanopore technology

Image: 3730xl GENETIC ANALYZER
3730xl GENETIC ANALYZER

Capillary electrophoresis platform for high throughput genetic analysis such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing 

Image: Illumina HiScan Reader
Illumina Hiscan Reader

Dedicated platform for high-precision array scanning of the Illumina BeadChips associated with GoldenGate® and Infinium® Genotyping assays 

Image: Axiom GeneTitan
Axiom GeneTitan

Hands-free dedicated platform for Affymetrix® High-Throughput (HT) Array Plates processing: from discoveries through genome-wide SNP genotyping 

Image: LIGHTCYCLER480 RT-PCR
LIGHTCYCLER480 RT-PCR

Medium-to high-throughput PCR platform that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation 

Image: ViiA7 RT-PCR
ViiA7 RT-PCR

Real Time qPCR platform for sensitive, specific detection and quantification of nucleic acid targets by 96 or 384 plates and TaqMan Array Cards 

Image: TapeStation 4150
TapeStation 4150

Automated electrophoresis platforms dedicated for the quality control (QC) of RNA and DNA samples in next-generation sequencing (NGS), microarray and qPCR workflows 

Click HERE  to book a device.

Services

Sample preparation

Nucleic Acid extraction, purification and quantification (DNA, RNA, plasmid); HMW DNA extraction (Nanopore Minion); NGS Library preparation and quantification (RNA-seq, Full Shotgun, Amplicon)

Sequencing

SANGER Sequencing (PCR, Plasmid, BAC sequencing); Next Generation Sequencing: a) full shotgun gDNA and eDNA; b) transcriptomic and expression study; c) metabarcoding analysis (16S, ITS, COI, trnL, rbcL)

Genotyping

SSR Genotyping (MAB, MAS); SNP Genotyping (WGAS); Genotyping by sequencing (GBS)

Click HERE  to book a service.

Applications

Agriculture

• Genome projects: plants, fungi, bacteria and virus;
• Molecular markers development;
• Marker assisted selection and Marker assisted breeding;
• Gene expression study;
• Plant - Pathogen interaction study;

Ecology

• Metagenomics and metabarcoding study (water, air, soil, bioreactors);
• Small genomes projects: animals, yeast and bacteria;
• Plant and Animals DNA Barcoding;

Diet and food science

• Metagenomics and metabarcoding food fermentation study;
• DNA barcoding for food traceability;
• Biotechnology for food security;
• Gut microbiome study (human and animals)