Concast (PI Nicola Cologna)

(PSR PAT 2023-2027) The project introduces an innovative approach to the biocontrol of E. coli/STEC in raw-milk cheeses through the combined use of selected antagonistic lactic acid bacteria (LAB) strains and nanoemulsions of plant compounds/essential oils. Innovation lies in integrating microbiological and natural strategies into a single multifactorial system applied directly to traditional cheesemaking processes. Different application methods (in the starter culture, in the curd, or as a coating) will be compared to optimize efficacy and dosage under production conditions. The expected outcome is a natural, sustainable, and easily adaptable bio-protection method that enhances product safety without altering the typical characteristics of artisanal cheeses.

The project will include the following activities:

• Preliminary design activities/support reports carried out during 2023-2024 in preparation for the project; project management and coordination activities and analytical support activities for project actions;
• Control the development/growth of STEC E. coli in raw milk cheeses (without pasteurising the milk) by developing a multi-obstacle control strategy combining both chemical and physical process treatments (adapting the temperature of curd cooking) and bio-control tactics, i.e. microbial strains and plant extracts with proven antimicrobial capacity to STEC E. coli;
• Development of specific sampling plans, management and analysis of matrices (milk and curds), processing of data and implementation of new tools for risk assessment and verification of process hygiene criteria. Assessment of production flows and collection of information on the technological processes used in the work. Assessment of applicability in various dairies in the area.