(MIUR PRIN) Development of a transgene-free genome editing tool for clonally propagated fruit crop. We would like in this project (i) use and develop nanoparticles which transport across the plant cell membranes protein and DNA. (ii) Identify nanoparticles or nanobubbles that are highly efficient for plant cell internalization, and utilize these nanoparticles to deliver DNA, RNA, and Cas9-gRNA RNP to different plants tissue such as calli, somatic embryos, meristem or protoplast in a fruit tree species-independent manner.

FEM activity:
in vitro characterization of nanoparticule and nanobubble systems for the delivery of DNA and CRISPR/Cas9 machinery in apple